منابع مشابه
Ca 2 Scraps Local
Free [Ca ] inside the sarcoplasmic reticulum ([Ca ]SR) is difficult to measure yet critically important in controlling many cellular systems. In cardiac myocytes, [Ca ]SR regulates cardiac contractility. We directly measure [Ca ]SR in intact cardiac myocytes dynamically and quantitatively during beats, with high spatial resolution. Diastolic [Ca ]SR (1 to 1.5 mmol/L) is only partially depleted ...
متن کاملSubcellular Ca(2+) Dynamics.
The field of subcellular Ca(2+) homeostasis is evolving rapidly. In parallel with improvements in spatial and temporal resolution of Ca(2+) imaging techniques, new methods using the natural cell machinery to target Ca(2+)-sensitive proteins such as aequorin to precise intracellular locations promise superb specificity to measure [Ca(2+)] in defined subcellular environments.
متن کاملLocalized Ca 2 uncaging reveals polarized distribution of Ca 2 - sensitive Ca 2 release sites : mechanism of unidirectional Ca 2 waves
a 2 -induced Ca 2 release (CICR) plays an important role in the generation of cytosolic Ca 2 signals in many cell types. However, it is inherently difficult to distinguish experimentally between the contributions of messenger-induced Ca 2 release and CICR. We have directly tested the CICR sensitivity of different regions of intact pancreatic acinar cells using local uncaging of caged Ca 2 . In ...
متن کاملAdrenergic stimulation of rat resistance arteries affects Ca(2+) sparks, Ca(2+) waves, and Ca(2+) oscillations.
Confocal laser scanning microscopy and fluo 4 were used to visualize local and whole cell Ca(2+) transients within individual smooth muscle cells (SMC) of intact, pressurized rat mesenteric small arteries during activation of alpha1-adrenoceptors. A method was developed to record the Ca(2+) transients within individual SMC during the changes in arterial diameter. Three distinct types of "Ca(2+)...
متن کاملGenome-wide RNAi screen of Ca(2+) influx identifies genes that regulate Ca(2+) release-activated Ca(2+) channel activity.
Recent studies by our group and others demonstrated a required and conserved role of Stim in store-operated Ca(2+) influx and Ca(2+) release-activated Ca(2+) (CRAC) channel activity. By using an unbiased genome-wide RNA interference screen in Drosophila S2 cells, we now identify 75 hits that strongly inhibited Ca(2+) influx upon store emptying by thapsigargin. Among these hits are 11 predicted ...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 2014
ISSN: 0009-7330,1524-4571
DOI: 10.1161/circresaha.113.303112